Cell Density Calculator

Average Number of Cells per Square:
Average Volume of Square (mL):
Dilution Factor:

Cell Density (cell/mL):


About Cell Density Calculator (Formula)

The Cell Density Calculator is a tool that helps determine the concentration of cells in a given sample. The most commonly used method to measure cell density is by using a hemocytometer, a specialized microscope slide with a gridded chamber that allows for the counting of cells in a known volume.

The formula used to calculate cell density is as follows:


Where: CD = Cell density per mL ACSQ = Average number of cells per square on the hemocytometer DF = Dilution factor (if the sample has not been diluted, this value is 1) VC = Volume of 1 cell (mL)

The user inputs the average number of cells per square, the average volume of the square, and the dilution factor, then the calculator applies the above formula to find the cell density per mL.

It’s important to note that the calculation of cell density is affected by many factors, including the size, shape, and staining of the cells, and the accuracy of the measurement can vary depending on the skill and experience of the person counting the cells.

It’s important to validate the results with a control sample and to make sure the sample is homogeneous.

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