About Concentration from Absorbance Calculator (Formula)
A Concentration from Absorbance Calculator is a tool used in chemistry and analytical science to determine the concentration of a solute in a solution based on the measurement of its absorbance. Absorbance is the amount of light absorbed by a substance at a specific wavelength, typically measured using a spectrophotometer. The concentration from absorbance is calculated using the Beer-Lambert Law, which relates absorbance (A), concentration (C), molar absorptivity (ε), and path length (l) of the sample:
A = ε * C * l
Where:
- A is the absorbance of the sample.
- ε is the molar absorptivity, also known as the molar extinction coefficient, which is a constant specific to the substance and the wavelength of light used in the measurement.
- C is the concentration of the solute in the solution, typically measured in moles per liter (M).
- l is the path length, which is the distance the light travels through the sample solution, typically measured in centimeters (cm).
To determine the concentration (C) from absorbance (A), you can rearrange the formula:
C = A / (ε * l)
This formula is particularly useful in analytical chemistry, biochemistry, and various scientific fields for quantifying the concentration of a substance in a solution based on its absorbance at a specific wavelength. Spectrophotometers are commonly used instruments to measure absorbance, and the Concentration from Absorbance Calculator simplifies the process of converting absorbance values into concentration measurements, making it an essential tool for researchers and analysts.